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5 December 2013 11:26 am ,
Vol. 342 ,
An animal rights group known as the Nonhuman Rights Project filed lawsuits in three New York courts this week in an...
Researchers have been hot on the trail of the elusive Denisovans, a type of ancient human known only by their DNA and...
Thousands of scientists in the Russian Academy of Sciences (RAS) are about to lose their jobs as a result of the...
Dyslexia, a learning disability that hinders reading, hasn't been associated with deficits in vision, hearing, or...
Exotic, elusive, and dangerous, snakes have fascinated humankind for millennia. They can be hard to find, yet their...
Researchers have sequenced and analyzed the first two snake genomes, which represent two evolutionary extremes. The...
Snake venoms are remarkably complex mixtures that can stun or kill prey within minutes. But more and more researchers...
At age 30, Dutch biologist Freek Vonk has built up a respectable career as a snake scientist. But in his home country,...
- 5 December 2013 11:26 am , Vol. 342 , #6163
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Sabotaging Bacterial Resistance
5 August 1997 8:00 pm
The rise of bacteria resistant to antibiotics has left researchers scrambling to develop more powerful drugs. But in the latest issue of the Proceedings of the National Academy of Sciences, researchers report their first success with an unconventional strategy: tricking the upstart bacteria into scrapping their defenses.
Getting the bacteria to give up their resistance required some genetic sleight of hand. Sidney Altman and his colleagues at Yale University used one of the bacteria's own enzymes, called RNase P, to disable RNA made from genes that had been linked to drug resistance. They designed a short piece of DNA, called an external guide sequence (EGS), whose RNA binds to the RNA template used to make proteins that confer drug resistance. The EGS was slipped into the cell in a circle of DNA called a plasmid. Combined, these two pieces of RNA look like RNase P's normal target, so the enzyme grabs on and slices the drug resistance RNA in two. The EGS emerges unchanged and binds to other copies of the drug-resistant RNA template. Altman and his colleagues created EGSs specific to two antibiotics, chloramphenicol and ampicillin, and effectively resensitized a resistant Escherichia coli strain to both drugs.
If EGSs can be adapted to reverse drug resistance in human bacterial and viral infections, they could revitalize many old and relatively ineffective antibiotics, says Altman. Allen Goldberg, chair of Innovir, a New York-based biotech company that has licensed EGS technology, says researchers there have already had some success at EGS treatments of mice infected with a drug-resistant hepatitis virus. But researchers still have to find a safe and easy way to put an EGS into human cells, a feat that could take several years of work. "At this point, these are only pipe dreams," he says.
Mitchell Cohen, a microbiologist at the Centers for Disease Control and Prevention, in Atlanta, says that although he finds the EGS approach to drug resistance "very clever," in the long run, it may be no more effective than another new antibiotic. "This method doesn't get around the problem of selective pressure." Bacteria will probably evolve a way around it, he says.